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Developmental Biology

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JoVE Journal Developmental Biology
Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis
 

Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis

Article DOI: 10.3791/63778-v • 10:41 min • May 19th, 2022
May 19th, 2022 •
1, 1
1Department of Biological Sciences, Northern Illinois University

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The basement membrane is essential for tissue and organ morphogenesis during development. To better understand the mechanisms leading to proper placement of this structure, the protocol presented describes methods to visualize and characterize the intracellular trafficking and secretion of basement membrane proteins in epithelial cells using confocal and super-resolution microscopy.

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Confocal Imaging Super-resolution Imaging Polarized Intracellular Trafficking Secretion Basement Membrane Proteins Drosophila Oogenesis Technique High-resolution Imaging Endogenously-tagged Proteins Airyscan Microscopy Protocol Biological Systems Cell Culture Organoids Fixation Solution Quick Washes Long Washes Blocking Solution Primary Antibody Solution
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