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Analysis of Fecal Microbiota Dynamics in Lupus-Prone Mice Using a Simple, Cost-Effective DNA Isolation Method
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Analysis of Fecal Microbiota Dynamics in Lupus-Prone Mice Using a Simple, Cost-Effective DNA Isolation Method

Analysis of Fecal Microbiota Dynamics in Lupus-Prone Mice Using a Simple, Cost-Effective DNA Isolation Method

1,908 Views

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05:28 min

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May 02, 2022

DOI:

10.3791/63623-v

DOI:
10.3791/63623-v

•

05:28 min
May 02, 2022

•

13 Views
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1Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine,Virginia Polytechnic Institute and State University

Summary

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This protocol provides a simple, cost-effective DNA isolation method for the analysis of murine gut microbiota alterations during the development of autoimmune disease.

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Fecal MicrobiotaDNA IsolationCost-effectiveAutoimmune DiseaseMouse ModelGut Microbiota DynamicsLysis BufferPhenol-chloroform ExtractionIsopropanol Precipitation260/280 Ratio

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Cite this Article

Cabana Puig, X., Reilly, C. M., Luo, X. M. Analysis of Fecal Microbiota Dynamics in Lupus-Prone Mice Using a Simple, Cost-Effective DNA Isolation Method. J. Vis. Exp. (183), e63623, doi:10.3791/63623 (2022).

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