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A TNBS-Induced Rodent Model to Study the Pathogenic Role of Mechanical Stress in Crohn’s Disease
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
A TNBS-Induced Rodent Model to Study the Pathogenic Role of Mechanical Stress in Crohn’s Disease

A TNBS-Induced Rodent Model to Study the Pathogenic Role of Mechanical Stress in Crohn’s Disease

DOI:
10.3791/63499-v

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05:08 min

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March 01, 2022

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, , , ,
1Department of Internal Medicine,the University of Texas Medical Branch, 2Department of Pathology,the University of Texas Medical Branch

Capitoli

  • 00:04Introduction
  • 01:02Animal Preparation
  • 01:41Tissue Preparation
  • 03:01Results: Crohn’s-Like Colitis Induced by Intra-Colonic TNBS Instillation
  • 04:21Conclusion

Summary

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The present protocol describes the development of a Crohn's-like colitis model in rodents. Transmural inflammation leads to stenosis at the TNBS instillation site, and mechanical enlargement is observed in the segment proximal to the stenosis. These changes allow studying mechanical stress in colitis.

Tags

TNBS-induced ColitisRodent ModelCrohn’s DiseaseMechanical StressInflammationColonProximal SegmentHistologyMacroscopic Score

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