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Optische clearing van plantenweefsels voor fluorescentiebeeldvorming
JoVE Journal
Biologia
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JoVE Journal Biologia
Optical Clearing of Plant Tissues for Fluorescence Imaging
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Optische clearing van plantenweefsels voor fluorescentiebeeldvorming

4,398 Views

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04:55 min

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January 05, 2022

DOI:

10.3791/63428-v

DOI:
10.3791/63428-v

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04:55 min
January 05, 2022

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26 Views
, , , ,
1Institute of Transformative Bio-Molecules (ITbM),Nagoya University, 2Institute for Advanced Research (IAR),Nagoya University, 3Division of Biological Science, Graduate School of Science,Nagoya University, 4Department of Biological Sciences, Graduate School of Science,The University of Tokyo

Summary

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Hier wordt een methode beschreven om plantenweefsels transparant te maken met behoud van de stabiliteit van fluorescerende eiwitten. Deze techniek vergemakkelijkt diepe beeldvorming van geklaarde plantenweefsels zonder fysieke doorsnede.

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Cite this Article

Kurihara, D., Mizuta, Y., Nagahara, S., Sato, Y., Higashiyama, T. Optical Clearing of Plant Tissues for Fluorescence Imaging. J. Vis. Exp. (179), e63428, doi:10.3791/63428 (2022).

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