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立体定位手术方法 ，通过 大水池在小鼠中显微注射尾部脑干和上颈椎脊髓
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Neuroscienze
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JoVE Journal Neuroscienze
Stereotaxic Surgical Approach to Microinject the Caudal Brainstem and Upper Cervical Spinal Cord via the Cisterna Magna in Mice
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立体定位手术方法 ，通过 大水池在小鼠中显微注射尾部脑干和上颈椎脊髓

DOI:
10.3791/63344-v

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07:07 min

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January 21, 2022

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, , ,
1Beth Israel Deaconess Medical Center,Harvard Medical School

Capitoli

  • 00:04Introduction
  • 00:52Positioning of the Mouse in the Stereotaxic Frame
  • 02:04Surgery to Access the Cisterna Magna
  • 03:34Opening of the Cisternal Membrane and Identification of Landmarks and Zero Point
  • 04:11Injection of the Target
  • 04:45Closure of the Surgical Field
  • 05:10Results: Stereotaxic Surgical Approach in Mice
  • 06:14Conclusion

Summary

Traduzione automatica

Traduzione automatica

针对小鼠大脑部位的立体定向手术通常涉及通过颅骨进入，并由颅骨特征引导。在这里，我们概述了一种替代立体定位方法， 通过 大水箱靶向尾部脑干和上颈椎脊髓，该方法依赖于脑干特征的直接可视化。

Tags

Keywords: StereotaxicMicroinjectionCaudal BrainstemUpper Cervical Spinal CordCisterna MagnaMiceNeurosurgical TechniquePrecision DeliveryRestricted Regions Of InterestReproducible AccessNeuroscience Research

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