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Isolation of Cardiac and Vascular Smooth Muscle Cells from Adult, Juvenile, Larval and Embryonic Zebrafish for Electrophysiological Studies
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Biologia
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JoVE Journal Biologia
Isolation of Cardiac and Vascular Smooth Muscle Cells from Adult, Juvenile, Larval and Embryonic Zebrafish for Electrophysiological Studies

Isolation of Cardiac and Vascular Smooth Muscle Cells from Adult, Juvenile, Larval and Embryonic Zebrafish for Electrophysiological Studies

DOI:
10.3791/63225-v

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07:04 min

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February 09, 2022

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, , , ,
1Department of Cell Biology and Physiology and Center for the Investigation of Membrane Excitability Diseases,Washington University in St. Louis, 2Department of Genetics, Center for Molecular Medicine,University Medical Center Utrecht, Utrecht University

Capitoli

  • 00:05Introduction
  • 00:42Isolation of Atrium, Ventricle, and Bulbous Arteriosus from Adult, Juvenile, and Larval Zebrafish
  • 01:37Dissociation of Cardiomyocytes from Adult, Juvenile, and Larval Zebrafish for Electrophysiological Studies
  • 02:59Dissociation of Vascular Smooth Muscle Cells from Adult and Juvenile Zebrafish for Electrophysiological Studies
  • 04:14Isolation of Hearts from Embryonic Zebrafish for Electrophysiological Studies
  • 05:21Results: Action Potential Properties from Isolated Zebrafish Cardiomyocytes

Summary

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The present protocol describes the acute isolation of viable cardiac and vascular smooth muscle cells from adult, juvenile, larval, and embryonic zebrafish (Danio rerio), suitable for electrophysiological studies.

Tags

Keywords: Cardiac MyocytesVascular Smooth Muscle CellsZebrafishElectrophysiologyPatch ClampCell IsolationPerfusion BufferDigestion BufferStopping BufferCentrifugationTrituration

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