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הדמיה של קספאזות דלקתיות המושרות קרבה במקרופאגים שמקורם במונוציטים אנושיים
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Visualization of Inflammatory Caspases Induced Proximity in Human Monocyte-Derived Macrophages
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הדמיה של קספאזות דלקתיות המושרות קרבה במקרופאגים שמקורם במונוציטים אנושיים

2,454 Views

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08:41 min

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April 06, 2022

DOI:

10.3791/63162-v

DOI:
10.3791/63162-v

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08:41 min
April 06, 2022

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3 Views
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1Department of Pediatrics, Division of Hematology-Oncology, Department of Molecular and Cellular Biology, Texas Children’s Hospital William T. Shearer Center for Human Immunobiology,Baylor College of Medicine

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Inflammatory CaspasesProximityHuman Monocyte-derived MacrophagesVisualizationMolecular-levelPrimary Immune CellsOligomerizationMicroscopic MethodologiesTrypsin-EDTAPBSCell NumberHemocytometerReporter PlasmidCaspase BiFC PlasmidsNucleofectionElectroporation

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Bolívar, B. E., Bouchier-Hayes, L. Visualization of Inflammatory Caspases Induced Proximity in Human Monocyte-Derived Macrophages. J. Vis. Exp. (182), e63162, doi:10.3791/63162 (2022).

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