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Isolation, Propagation, and Identification of Bacterial Species with Hydrocarbon Metabolizing Properties from Aquatic Habitats
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Isolation, Propagation, and Identification of Bacterial Species with Hydrocarbon Metabolizing Properties from Aquatic Habitats

Isolation, Propagation, and Identification of Bacterial Species with Hydrocarbon Metabolizing Properties from Aquatic Habitats

DOI:
10.3791/63101-v

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10:03 min

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December 07, 2021

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1Department of Life Sciences, School of Natural Sciences,Shiv Nadar University

Capitoli

  • 00:04Introduction
  • 00:52Sample Collection, Processing, and Analysis
  • 03:12Degradation of Hydrocarbons
  • 05:04Genomic DNA Isolation of the Pure Culture
  • 06:4916S rRNA Sequencing
  • 08:07Results: Isolation and Characterization of Hydrocarbon Degrading Bacteria from Aquatic Habitats
  • 09:11Conclusion

Summary

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We present the process of isolating, propagating, and characterizing hydrocarbon-degrading bacteria from aquatic habitats. The protocol outlines bacterial isolation, identification by the 16S rRNA method, and testing of their hydrocarbon-degrading potential. This article would help researchers in characterizing microbial biodiversity in environmental samples, and specifically screen for microbes with bioremediation potential.

Tags

Keywords: Hydrocarbon-degrading MicroorganismsAquatic HabitatsIsolationCultivationIdentificationBacterial SpeciesWater SampleNutrient MediaDilutionColony ScreeningGram StainingGlycerol StocksHydrocarbon Metabolism

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