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Probing Structural and Dynamic Properties of Trafficking Subcellular Nanostructures by Spatiotemporal Fluctuation Spectroscopy
JoVE Journal
Biologia
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JoVE Journal Biologia
Probing Structural and Dynamic Properties of Trafficking Subcellular Nanostructures by Spatiotemporal Fluctuation Spectroscopy

Probing Structural and Dynamic Properties of Trafficking Subcellular Nanostructures by Spatiotemporal Fluctuation Spectroscopy

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08:17 min

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August 16, 2021

DOI:

10.3791/62790-v

DOI:
10.3791/62790-v

•

08:17 min
August 16, 2021

•

2 Views
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1NEST Laboratory,Scuola Normale Superiore, 2Center for Nanotechnology Innovation@NEST CNI@NEST

Summary

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Imaging-derived mean square displacement (iMSD) analysis is applied to macropinosomes to highlight their intrinsic time-evolving nature in terms of structural and dynamic properties. Macropinosomes are then compared to insulin secretory granules (ISGs) as a reference for subcellular structures with time-invariant average structural/dynamic properties.

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Cite this Article

Ferri, G., Azzarello, F., D'Autilia, F., Cardarelli, F. Probing Structural and Dynamic Properties of Trafficking Subcellular Nanostructures by Spatiotemporal Fluctuation Spectroscopy. J. Vis. Exp. (174), e62790, doi:10.3791/62790 (2021).

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