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An Intestinal Gut Organ Culture System for Analyzing Host-Microbiota Interactions
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
An Intestinal Gut Organ Culture System for Analyzing Host-Microbiota Interactions

An Intestinal Gut Organ Culture System for Analyzing Host-Microbiota Interactions

DOI:
10.3791/62779-v

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05:27 min

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June 30, 2021

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, , , ,
1The Goodman Faculty of Life Sciences,Bar-Ilan University, 2Bar-Ilan Institute of Nanotechnology and Advanced Materials,Bar-Ilan University

Capitoli

  • 00:05Introduction
  • 00:53Experiment Preparation
  • 02:02Experiment Setup Preparation
  • 02:25Organ Cultures
  • 03:36Results: Assessment of Intestinal Structure and Host-Microbiota Interactions Using Gut Organ Culture
  • 04:46Conclusion

Summary

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This article presents a unique method for analyzing host-microbiome interactions using a novel gut organ culture system for ex vivo experiments.

Tags

Gut Organ CultureIntestinal PhysiologyHost-microbiota InteractionsIn Vitro AssayIn Vivo ExperimentPDMSIntestinal FlushMouse Dissection

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