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A High Throughput Microplate Feeder Assay for Quantification of Consumption in Drosophila
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A High Throughput Microplate Feeder Assay for Quantification of Consumption in Drosophila
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June 14, 2021
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The microplate feeder assay offers an economical, high throughput method for quantifying liquid food consumption in Drosophila. A 3D-printed device connects a 96-well microplate in which flies are housed to a 1536-well microplate from which flies consume a feeding solution with a tracer dye. The solution volume decline is measured spectrophotometrically.
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Walters, J. D., Hatfield, J. S., Baker, B. B., Mackay, T. F. C., Anholt, R. R. H. A High Throughput Microplate Feeder Assay for Quantification of Consumption in Drosophila. J. Vis. Exp. (172), e62771, doi:10.3791/62771 (2021).
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