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单纯疱疹病毒的生长、净化和滴定
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Growth, Purification, and Titration of Oncolytic Herpes Simplex Virus
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单纯疱疹病毒的生长、净化和滴定

DOI:
10.3791/62677-v

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06:14 min

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May 13, 2021

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, , , ,
1Department of Immunotherapeutics and Biotechnology, Jerry H. Hodge School of Pharmacy,Texas Tech University Health Sciences Center, 2Molecular Neurosurgery Laboratory and the Brain Tumor Research Center, Department of Neurosurgery,Massachusetts General Hospital and Harvard Medical School

Capitoli

  • 00:04Introduction
  • 01:06Oncolytic Herpes Simplex Virus (oHSV) Growth
  • 02:46Oncolytic Herpes Simplex Virus (oHSV) Purification
  • 05:10Results: Microscopic Analysis of Vero Cells Infected with oHSV
  • 05:44Conclusion

Summary

Traduzione automatica

Traduzione automatica

在本手稿中，我们描述了一个简单的方法的生长，纯化和滴定肿瘤疱疹简单病毒的药物前使用。

Tags

Oncolytic Herpes Simplex VirusCancer ImmunotherapyVirus PropagationVirus PurificationVirus TitrationBiosafety Level TwoVirus StockAnticancer Immune ResponseVirus-based Cancer ImmunotherapyWild-type Herpes Simplex VirusGenetically Engineered Herpes Simplex VirusT-150 Square Centimeter FlasksDPBSIFCSDMEMCell ScraperCentrifugationVirus BufferBenzonase NucleaseVirus Titer

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