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Imaging Dendritic Spines in Caenorhabditis elegans
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Neuroscienze
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JoVE Journal Neuroscienze
Imaging Dendritic Spines in Caenorhabditis elegans

Imaging Dendritic Spines in Caenorhabditis elegans

DOI:
10.3791/62676-v

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09:14 min

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September 27, 2021

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,
1Department of Cell and Developmental Biology,Vanderbilt University, 2Program of Neuroscience,Vanderbilt University

Capitoli

  • 00:05Introduction
  • 00:53Determination of Dorsal D (DD) Dendritic Spines Structure
  • 02:44Assessing Activation of DD Dendritic Spines by Presynaptic Cholinergic Signaling
  • 07:06Results: Imaging and Assessing the Function of DD Dendritic Spines in Caenorhabditis elegans
  • 08:37Conclusion

Summary

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Dendritic spines are important cellular features of the nervous system. Here live imaging methods are described for assessing the structure and function of dendritic spines in C. elegans. These approaches support the development of mutant screens for genes that define dendritic spine shape or function.

Tags

Dendritic SpinesCaenorhabditis ElegansGABA NeuronsImmobilizationSuper-resolution MicroscopyNyquist Acquisition3D-deconvolutionSpine DensitySpine ClassificationCalcium Sensor GCaMPChrimsonAll-trans-retinal

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