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Immunologia e infezioni

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通过荧光原位杂交、凝集素染色和成像可视化肠道微生物群与宿主的相互作用

JoVE Journal
Immunologia e infezioni

This content is Free Access.

JoVE Journal Immunologia e infezioni

Visualization of Gut Microbiota-host Interactions via Fluorescence In Situ Hybridization, Lectin Staining, and Imaging

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通过荧光原位杂交、凝集素染色和成像可视化肠道微生物群与宿主的相互作用

6,596 Views

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09:31 min

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July 09, 2021

DOI:

10.3791/62646-v

DOI:

10.3791/62646-v

•

09:31 min

July 09, 2021

•

26 Views

Katharine M. Ng^1,2, Carolina Tropini^1,2,3

¹School of Biomedical Engineering,University of British Columbia, ²Department of Microbiology and Immunology,University of British Columbia, ³Humans and the Microbiome Program,Canadian Institute for Advanced Research (CIFAR)

Summary

这种简化的实验方案详细介绍了检测和成像复杂组织样品中细菌的工作流程，从固定组织到用荧光原位杂交染色微生物。

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