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OnePot PURE Cell-Free System
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Bioingegneria
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JoVE Journal Bioingegneria
OnePot PURE Cell-Free System

OnePot PURE Cell-Free System

DOI:
10.3791/62625-v

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08:25 min

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June 23, 2021

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, , ,
1Institute of Bioengineering, School of Engineering,École Polytechnique Fédérale de Lausanne

Capitoli

  • 00:04Introduction
  • 00:54OnePot Protein Solution Expression and Purification
  • 05:44OnePot PURE Reaction
  • 06:51Results: OnePot Protein Purification and Overexpression
  • 07:44Conclusion

Summary

Traduzione automatica

Traduzione automatica

We present a fast and cost-effective method to produce the recombinant PURE cell-free TX-TL system using standard laboratory equipment.

Tags

AI Writing AssistantCell-free Protein ExpressionPURE SystemOnePot MethodRecombinant Protein PurificationStreamlined Protein ProductionTime-efficient SystemCost-effective PlatformSynthetic Cell-free Biology

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