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密度梯度超速离心研究哺乳动物细胞内吞循环
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JoVE Journal Biochimica
Density Gradient Ultracentrifugation for Investigating Endocytic Recycling in Mammalian Cells
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密度梯度超速离心研究哺乳动物细胞内吞循环

DOI:
10.3791/62621-v

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05:13 min

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June 30, 2021

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, ,
1School of Life Sciences,The Chinese University of Hong Kong

Capitoli

  • 00:05Introduction
  • 00:33Mammalian Cell Culture, Transfection and Cell Harvest
  • 02:07Density Gradient Column Preparation
  • 02:46Fractionation and Recovery of Recycling Endosome-Containing Fraction
  • 03:17Results: Density Gradient Ultracentrifugation for Investigating Endocytic Recycling in Mammalian Cells
  • 04:47Conclusion

Summary

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本文旨在提出一种利用蔗糖密度梯度超速离心制备哺乳动物细胞内体回收的方案。

Tags

Density Gradient UltracentrifugationEndocytic RecyclingMammalian CellsProtein TraffickingRecycling EndosomesCell FractionationSucrose GradientPost-nuclear SupernatantHomogenization BufferDounce HomogenizerProtease InhibitorPhosphatase Inhibitor

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