This content is Free Access.
1Vanderbilt Eye Institute,Vanderbilt University Medical Center, 2Department of Cell and Developmental Biology,Vanderbilt University, 3Department of Biochemistry,Vanderbilt University, 4Division of Clinical Pharmacology, Department of Medicine,Vanderbilt University Medical Center, 5Division of Hematology and Oncology,Indiana University School of Medicine, 6Department of Molecular Physiology and Biophysics,Vanderbilt University
Capitoli
- 00:05Introduction
- 00:54Immunofluorescence
- 03:21Negative Controls and Competitive Assays
- 04:37Results: Detection of IsoLGs in Hypertensive and Normotensive Mice and Human Tissues
- 06:08Conclusion
Este artigo fornece uma metodologia detalhada para a dosagem de isolevuglandinas em tecidos por imunofluorescência usando o anticorpo ScFv D11 conjugado à fosfatase alcalina. Modelos de hipertensão em camundongos e humanos são usados para explicar os procedimentos passo a passo e os princípios fundamentais associados à dosagem de isolevuvolina em amostras de tecido.
