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Identification of Antibacterial Immunity Proteins in Escherichia coli using MALDI-TOF-TOF-MS/MS and Top-Down Proteomic Analysis

JoVE Journal
Chimica

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JoVE Journal Chimica

Identification of Antibacterial Immunity Proteins in Escherichia coli using MALDI-TOF-TOF-MS/MS and Top-Down Proteomic Analysis

Identification of Antibacterial Immunity Proteins in Escherichia coli using MALDI-TOF-TOF-MS/MS and Top-Down Proteomic Analysis

2,895 Views

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09:26 min

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May 23, 2021

DOI:

10.3791/62577-v

DOI:

10.3791/62577-v

•

09:26 min

May 23, 2021

•

3 Views

Clifton K. Fagerquist, Ernesto Rojas

¹Produce Safety & Microbiology, Western Regional Research Center,Agricultural Research Service, U.S. Department of Agriculture

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Escherichia Coli Antibacterial Immunity Proteins MALDI-TOF-TOF-MS/MS Top-down Proteomics Mass Spectrometry Bacterial Protein Identification Antibiotic Induction Sample Preparation HPLC-grade Water Sinapinic Acid MS Linear Mode Acquisition MS/MS Reflectron Mode Acquisition Precursor Mass Isolation Width CID Metastable Suppressor

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