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Análise da Restrição de SAMHD1 por Citometria de Fluxo em Células U937 Mieloides Humanas
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Analysis of SAMHD1 Restriction by Flow Cytometry in Human Myeloid U937 Cells

Análise da Restrição de SAMHD1 por Citometria de Fluxo em Células U937 Mieloides Humanas

1,839 Views

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09:43 min

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June 13, 2021

DOI:

10.3791/62502-v

DOI:
10.3791/62502-v

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09:43 min
June 13, 2021

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14 Views
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1Retrovirus-Host Interactions Laboratory,The Francis Crick Institute, 2Retroviral Replication Laboratory,The Francis Crick Institute, 3Sidney Sussex College, Department of Medicine,University of Cambridge

Tags

SAMHD1Flow CytometryU937 CellsHIV InfectionMacrophagesCellular FactorsMulticolor Flow CytometryCytometerFlow RateForward ScatterSide ScatterYFPRFPCompensation Controls

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Ordonez, P., Bishop, K. N., Stoye, J. P., Groom, H. C. T. Analysis of SAMHD1 Restriction by Flow Cytometry in Human Myeloid U937 Cells. J. Vis. Exp. (172), e62502, doi:10.3791/62502 (2021).

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