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Generation of Zebrafish Larval Xenografts and Tumor Behavior Analysis
JoVE Journal
Ricerca sul cancro
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JoVE Journal Ricerca sul cancro
Generation of Zebrafish Larval Xenografts and Tumor Behavior Analysis

Generation of Zebrafish Larval Xenografts and Tumor Behavior Analysis

10,461 Views

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12:08 min

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June 19, 2021

DOI:

10.3791/62373-v

DOI:
10.3791/62373-v

•

12:08 min
June 19, 2021

•

57 Views
, ,
1Champalimaud Centre for the Unknown, Champalimaud Foundation, 2Instituto Gulbenkian de Ciência

Summary

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Here, we provide a step-by-step protocol, with tips to generate xenografts and guidelines for tumor behavior analysis, whole-mount immunofluorescence, and confocal imaging quantification.

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Zebrafish XenograftsRicerca sul cancroLarva ModelProliferationAngiogenesisMetastasisTumor MicroenvironmentAnti-cancer TherapiesPatient-derived CellsCell InjectionIn-vitro ConfluenceZebrafish EmbryosAgarose PlatesMicroinjection

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Cite this Article

Martinez-Lopez, M., Póvoa, V., Fior, R. Generation of Zebrafish Larval Xenografts and Tumor Behavior Analysis. J. Vis. Exp. (172), e62373, doi:10.3791/62373 (2021).

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