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JoVE Journal Neuroscience
A Time-Efficient Fluorescence Spectroscopy-Based Assay for Evaluating Actin Polymerization Status in Rodent and Human Brain Tissues
 

A Time-Efficient Fluorescence Spectroscopy-Based Assay for Evaluating Actin Polymerization Status in Rodent and Human Brain Tissues

Article DOI: 10.3791/62268-v • 06:54 min • June 3rd, 2021
June 3rd, 2021 •
1, 1
1Department of Anatomy, School of Biomedical Sciences, University of Otago

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We report a simple, time-efficient and high-throughput fluorescence spectroscopy-based assay for the quantification of actin filaments in ex vivo biological samples from brain tissues of rodents and human subjects.

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Keywords: Actin Cytoskeleton F-actin G-actin Polymerization Fluorescence Spectroscopy Phalloidin Synaptic Terminals Synaptosomes Synaptoneurosomes Brain Tissue Neuropathology Neuronal Physiology
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