The sterile insect technique (SIT) is used to control specific, medically important mosquito populations that may be resistant to chemical controls. Here, we describe a method of mass rearing and preparation of sterile male mosquitoes for release in an operational SIT program targeting the Aedes aegypti mosquito.
Moreno, B. J., Aldridge, R. L., Britch, S. C., Bayer, B. E., Kline, J., Hahn, D. A., Chen, C., Linthicum, K. J. Preparing Irradiated and Marked Male Aedes aegypti Mosquitoes for Release in an Operational Sterile Insect Technique Program. J. Vis. Exp. (169), e62260, doi:10.3791/62260 (2021).