Rivista
/
Neuroscienze
/
In vivo Calcium Imaging in Mouse Inferior Olive
JoVE Journal
Neuroscienze
Author Produced
This content is Free Access.
JoVE Journal Neuroscienze
In vivo Calcium Imaging in Mouse Inferior Olive

In vivo Calcium Imaging in Mouse Inferior Olive

DOI:
10.3791/62222-v

•

08:58 min

•

June 10, 2021

•

, ,
1Neuronal Rhythms in Movement unit,OIST

Capitoli

  • 00:00Introduction
  • 00:57Protocol
  • 01:45Administration of Anesthesia and Preparation of the Mouse for the Surgery
  • 02:42Tracheotomy and Intubation
  • 05:02Exposing the Brainstem
  • 06:42Calcium Imagin
  • 07:55Representative Results
  • 08:20Conclusion

Summary

Traduzione automatica

Traduzione automatica

We present a protocol to expose the brainstem of adult mouse from the ventral side. By using a gradient-refractive index lens with a miniature microscope, calcium imaging can be used to examine the activity of inferior olive neural somata in vivo.

Tags

Keywords: In VivoCalcium ImagingMouseInferior OliveBrain StemGRIN LensesNeuronal ActivityCalcium SensorsSpatial-temporal Activity PatternsInput IntegrationSurgical SkillsTracheotomyKetamineIsofluraneAnesthesiaBlunt DissectionTracheaSternothyroid Muscle

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/62222?article_type=v

Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation
check_url/it/62222?article_type=v

Functional Neuroimaging Using Ultrasonic Blood-brain Barrier Disruption and Manganese-enhanced MRI
check_url/it/62222?article_type=v

In vivo Neuronal Calcium Imaging in C. elegans
check_url/it/62222?article_type=v

In Vivo Functional Brain Imaging Approach Based on Bioluminescent Calcium Indicator GFP-aequorin
check_url/it/62222?article_type=v

In Vivo Calcium Imaging of Lateral-line Hair Cells in Larval Zebrafish
check_url/it/62222?article_type=v

Successful In vivo Calcium Imaging with a Head-Mount Miniaturized Microscope in the Amygdala of Freely Behaving Mouse
check_url/it/62222?article_type=v

Transpupillary Two-Photon In Vivo Imaging of the Mouse Retina
check_url/it/62222?article_type=v

In vivo Calcium Imaging of Mouse Geniculate Ganglion Neuron Responses to Taste Stimuli
check_url/it/62222?article_type=v

Brain Pericyte Calcium and Hemodynamic Imaging in Transgenic Mice In Vivo
check_url/it/62222?article_type=v

In Vivo Calcium Imaging of Neuronal Ensembles in Networks of Primary Sensory Neurons in Intact Dorsal Root Ganglia

Read Article