Rivista
/
Biologia
/
In vivo Measurement of Knee Extensor Muscle Function in Mice
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
In vivo Measurement of Knee Extensor Muscle Function in Mice

In vivo Measurement of Knee Extensor Muscle Function in Mice

DOI:
10.3791/62211-v

•

08:29 min

•

March 04, 2021

•

, , , , ,
1Department of Athletic Training and Clinical Nutrition,University of Kentucky, 2Center for Muscle Biology,University of Kentucky, 3Department of Physical Therapy,East Carolina University, 4Kinesiology and Health Promotion Graduate Program,University of Kentucky, 5Biomotion Lab, College of Health Sciences,University of Kentucky, 6Aurora Scientific, 7Department of Physical Therapy, College of Health Sciences,University of Kentucky

Capitoli

  • 00:04Introduction
  • 01:00Equipment Setup
  • 01:33Software Setup
  • 02:29Mouse Setup
  • 03:24Electrode Placement
  • 04:18Determination of Optimal Current
  • 05:00Torque-Frequency Experiment to Determine Peak Isometric Tetanic Torque
  • 06:10Results: In Vivo Measurement of Knee Extensor Muscle Function in Mice
  • 07:43Conclusion

Summary

Traduzione automatica

Traduzione automatica

Quantification of knee extensor maximal strength is imperative to understand functional adaptations to aging, disease, injury, and rehabilitation. We present a novel method to repeatedly measure in vivo knee extension isometric peak tetanic torque.

Tags

Knee ExtensorMuscle FunctionMiceIsometric Peak Tetanic TorqueNoninvasive AssessmentQuadricep StrengthPre-clinical ModelsOsteoarthritisKnee InjuryElectrodesStimulationTwitchTorque FrequencyAnesthesiaSedationShavingClamp

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

In vivo Micro-circulation Measurement in Skeletal Muscle by Intra-vital Microscopy

Ex Vivo Assessment of Contractility, Fatigability and Alternans in Isolated Skeletal Muscles

Evaluation of Muscle Function of the Extensor Digitorum Longus Muscle Ex vivo and Tibialis Anterior Muscle In situ in Mice

Force Measurement During Contraction to Assess Muscle Function in Zebrafish Larvae

Measurement of Smooth Muscle Function in the Isolated Tissue Bath-applications to Pharmacology Research

Functional Reconstitution and Channel Activity Measurements of Purified Wildtype and Mutant CFTR Protein

Murine Aortic Crush Injury: An Efficient In Vivo Model of Smooth Muscle Cell Proliferation and Endothelial Function

A Rapid Automated Protocol for Muscle Fiber Population Analysis in Rat Muscle Cross Sections Using Myosin Heavy Chain Immunohistochemistry

An Ex Vivo Tissue Culture Model of Cartilage Remodeling in Bovine Knee Explants

Measurement of Insulin- and Contraction-Stimulated Glucose Uptake in Isolated and Incubated Mature Skeletal Muscle from Mice

Read Article