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Generación y expansión de cardiomiocitos humanos a partir de células mononucleares de sangre periférica de pacientes
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Generación y expansión de cardiomiocitos humanos a partir de células mononucleares de sangre periférica de pacientes
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05:38 min
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February 12, 2021
DOI:
1Center for Cardiovascular Research, The Abigail Wexner Research Institute,Nationwide Children’s Hospital, 2The Heart Center,Nationwide Children’s Hospital, 3Department of Physiology and Cell Biology,The Ohio State University College of Medicine, 4Department of Anatomy,The Ohio State University College of Medicine, 5MCDB Graduate Program,The Ohio State University, 6Department of Pediatrics,The Ohio State University College of Medicine
Summary
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Aquí, presentamos un protocolo para generar y expandir de manera robusta los cardiomiocitos humanos a partir de células mononucleares de sangre periférica del paciente.
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Ye, S., Wan, X., Su, J., Patel, A., Justis, B., Deschênes, I., Zhao, M. Generation and Expansion of Human Cardiomyocytes from Patient Peripheral Blood Mononuclear Cells. J. Vis. Exp. (168), e62206, doi:10.3791/62206 (2021).
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