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发育生物学
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Generation and Expansion of Human Cardiomyocytes from Patient Peripheral Blood Mononuclear Cells
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JoVE 杂志 发育生物学
Generation and Expansion of Human Cardiomyocytes from Patient Peripheral Blood Mononuclear Cells

Generation and Expansion of Human Cardiomyocytes from Patient Peripheral Blood Mononuclear Cells

DOI:
10.3791/62206-v

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05:38 min

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February 12, 2021

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, , , , , ,
1Center for Cardiovascular Research, The Abigail Wexner Research Institute,Nationwide Children’s Hospital, 2The Heart Center,Nationwide Children’s Hospital, 3Department of Physiology and Cell Biology,The Ohio State University College of Medicine, 4Department of Anatomy,The Ohio State University College of Medicine, 5MCDB Graduate Program,The Ohio State University, 6Department of Pediatrics,The Ohio State University College of Medicine

Chapters

  • 00:04Introduction
  • 01:08Human Induced Pluripotent Stem Cell (iPSC) Maintenance and Passaging
  • 01:55Chemically Defined Cardiomyocyte (CM) Differentiation
  • 02:57Results: Representative iPSC-CM Differentiation and Function
  • 04:51Conclusion

Summary

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Here, we present a protocol to robustly generate and expand human cardiomyocytes from patient peripheral blood mononuclear cells.

Tags

Cardiomyocyte GenerationPeripheral Blood Mononuclear CellsIPSC ReprogrammingCardiovascular Disease ModelingCardiac Toxicity TestingDifferentiation ProtocolCell CultureSendai VirusPBMCE8 MediumCardiomyocyte Differentiation Media

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