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用于研究呼吸道病毒感染期间先天免疫细胞活化的非接触式共培养模型
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07:36 min
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February 28, 2021
DOI:
1Department of Otolaryngology, Yong Loo Lin School of Medicine,National University of Singapore, 2Department of Microbiology and Immunology, Yong Loo Lin School of Medicine,National University of Singapore, 3Haartman Institute, Medicum,University of Helsinki, 4Department of Otolaryngology, Head and Neck Surgery, National University Hospital,National University Health System, 5Agency for Science, Technology and Research (A*STAR),Singapore Immunology Network (SIgN), 6NUHS Infectious Diseases Translational Research Program, Yong Loo Lin School of Medicine,National University of Singapore
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该协议详细研究了病毒感染的鼻上皮细胞与先天细胞活化之间的早期相互作用。免疫细胞的单个亚群可以根据其对病毒感染的反应而激活来区分。然后可以进一步研究它们以确定它们对早期抗病毒反应的影响。
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Lew, Z. Z. R., Liu, J., Ong, H. H., Tan, V. J., Luukkainen, A., Ong, Y. K., Thong, M., Puan, K. J., Chow, V. T. K., Tan, K. S., Wang, D. Y. Contact-Free Co-Culture Model for the Study of Innate Immune Cell Activation During Respiratory Virus Infection. J. Vis. Exp. (168), e62115, doi:10.3791/62115 (2021).
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