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Contact-Free Co-Culture Model for the Study of Innate Immune Cell Activation During Respiratory Virus Infection
1Department of Otolaryngology, Yong Loo Lin School of Medicine,National University of Singapore, 2Department of Microbiology and Immunology, Yong Loo Lin School of Medicine,National University of Singapore, 3Haartman Institute, Medicum,University of Helsinki, 4Department of Otolaryngology, Head and Neck Surgery, National University Hospital,National University Health System, 5Agency for Science, Technology and Research (A*STAR),Singapore Immunology Network (SIgN), 6NUHS Infectious Diseases Translational Research Program, Yong Loo Lin School of Medicine,National University of Singapore
Chapters
- 00:04Introduction
- 00:50hNEC Viral Infection and hNEC + PBMCs Co-Culture
- 03:30Harvesting of PBMCs 48/72 h Post-Viral Infection
- 05:31Results: Activation of T Cells Following Influenza Infection
- 06:56Conclusion
This protocol details an investigation of the early interactions between virally infected nasal epithelial cells and innate cell activation. Individual subsets of immune cells can be distinguished based on their activation in response to viral infections. They can then be further investigated to determine their effects on early antiviral responses.
