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使用上格拉夫方法估计植物生物质的晶体纤维素含量
JoVE Journal
Ambiente
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JoVE Journal Ambiente
Estimation of Crystalline Cellulose Content of Plant Biomass using the Updegraff Method
DOI:

12:34 min

May 15, 2021

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Capitoli

  • 00:00Introduction
  • 01:12Phase I: Preparation of Samples
  • 02:02Phase II: Preparation of the Alcohol Insoluble Crude Cell Wall
  • 04:52Phase III: Removal of Hemicellulose and Lignin using Updegraff Reagent
  • 07:10Phase IV: Acid Hydrolysis of Cellulose into Glucose Monomers
  • 07:49Phase V: Anthrone Assay to Estimate the Glucose Concentration of Samples and Glucose Standard
  • 10:04Representative Results & Conclusion

Summary

Traduzione automatica

上格拉夫法是纤维素估计使用最广泛的方法。本次演示的主要目的是提供详细的上格拉夫协议,用于估算植物生物质样本中的纤维素含量。

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