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使用从纤维细胞直接转换为神经元祖先细胞和分化为星形细胞的神经系统疾病体外建模

JoVE Journal
Neuroscienze
Author Produced

This content is Free Access.

JoVE Journal Neuroscienze

In vitro Modeling for Neurological Diseases using Direct Conversion from Fibroblasts to Neuronal Progenitor Cells and Differentiation into Astrocytes

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使用从纤维细胞直接转换为神经元祖先细胞和分化为星形细胞的神经系统疾病体外建模

DOI:

10.3791/62016-v

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11:42 min

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June 10, 2021

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Cassandra N. Dennys, Julieth A. Sierra-Delgado, Shrestha Sinha Ray, Annalisa M. Hartlaub, Florence S. Roussel, Yacidzohara Rodriguez, Kathrin Meyer²

¹Center for Gene Therapy,The Research Institute at Nationwide Children’s Hospital, ²College of Medicine, The Ohio State University

Capitoli

00:00Introduction
01:01Direct Conversion of Human Fibroblasts to Neuronal Progenitor Cells
04:21Conversion and iNPC Splitting
06:42Generating Induced Astrocytes From NPCs
07:43Astrocyte Differentiation From Frozen iNPC Stocks
08:51Results: Growth and Characterization of Cells Throughout Conversion Process
10:53Conclusion

Summary

Traduzione automatica

我们描述了一个将人类皮肤衍生的成纤维细胞重新编程为诱导神经元祖先细胞（iNPC）的协议，以及它们随后分化成诱导的星形细胞（iAs）的协议。这种方法可快速、可重复地大量生成 iNC 和 iAs。

Tags

Keywords: In Vitro Modeling Neurological Diseases Fibroblasts Neuronal Progenitor Cells Astrocytes Direct Conversion Skin Biopsy Retroviral Vectors Oct3/4 Klf4 Sox2 C-Myc Transduction Neurodegenerative Diseases Cell Culture Co-cultures Disease Mechanisms Medication Testing

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