The goal of this protocol is to provide detailed guidance on the sample preparation when planning for experiments using MALDI MSI to maximize metabolic and molecular detection in biological samples.
Veerasammy, K., Chen, Y. X., Sauma, S., Pruvost, M., Dansu, D. K., Choetso, T., Zhong, T., Marechal, D., Casaccia, P., Abzalimov, R., He, Y. Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging. J. Vis. Exp. (166), e62008, doi:10.3791/62008 (2020).