Rivista
/
Immunologia e infezioni
/
A Contemporary Warming/Restraining Device for Efficient Tail Vein Injections in a Murine Fungal Sepsis Model
JoVE Journal
Immunologia e infezioni
This content is Free Access.
JoVE Journal Immunologia e infezioni
A Contemporary Warming/Restraining Device for Efficient Tail Vein Injections in a Murine Fungal Sepsis Model

A Contemporary Warming/Restraining Device for Efficient Tail Vein Injections in a Murine Fungal Sepsis Model

DOI:
10.3791/61961-v

•

06:02 min

•

November 06, 2020

•

, , ,
1Center for Oral and Craniofacial Biology,Louisiana State University Health-School of Dentistry, 2Department of Microbiology and Immunology,Tulane University School of Medicine

Capitoli

  • 00:04Introduction
  • 00:47Setting Up of the Warming and Restraint Device
  • 01:33Tail Vein Injection
  • 04:03Results: Sepsis Mortality vs. Vaccine-Mediated Protection Following a Lethal Challenge with Candida albicans
  • 05:23Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here, we present an effective and efficient method for rodent tail vein injections using a uniquely designed warming/restraining device. By streamlining the initiation of vasodilation and restraining processes, this protocol allows accurate and timely intravenous injections of large groups of animals with minimal distress.

Tags

Warming DeviceRestraining DeviceTail Vein InjectionMurineFungal Sepsis ModelIntravenous InjectionVasodilationHeat TreatmentAnimal HandlingProtocolVisual Demonstration

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Colon Ascendens Stent Peritonitis (CASP) – a Standardized Model for Polymicrobial Abdominal Sepsis

Induction of Graft-versus-host Disease and In Vivo T Cell Monitoring Using an MHC-matched Murine Model

Cecal Ligation and Puncture-induced Sepsis as a Model To Study Autophagy in Mice

Transplantation of Tail Skin to Study Allogeneic CD4 T Cell Responses in Mice

A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors

A Murine Model of Group B Streptococcus Vaginal Colonization

Stenosis of the Inferior Vena Cava: A Murine Model of Deep Vein Thrombosis

Deep Vein Thrombosis Induced by Stasis in Mice Monitored by High Frequency Ultrasonography

In Vivo Imaging of Reactive Oxygen Species in a Murine Wound Model

A Controlled Mouse Model for Neonatal Polymicrobial Sepsis

Read Article