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An Antegrade Perfusion Method for Cardiomyocyte Isolation from Mice
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May 19, 2021
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We developed a simple method for isolating high quality individual mouse heart cells by the antegrade perfusion technique. This method is Langendorff-free and useful for isolating ventricular and atrial myocytes or interstitial cells, such as cardiac fibroblasts or progenitors.
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Omatsu-Kanbe, M., Fukunaga, R., Mi, X., Matsuura, H. An Antegrade Perfusion Method for Cardiomyocyte Isolation from Mice. J. Vis. Exp. (171), e61866, doi:10.3791/61866 (2021).
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