Rivista
/
Immunologia e infezioni
/
T- en B-celreceptor immuunrepertoireanalyse met behulp van next-generation sequencing
JoVE Journal
Immunologia e infezioni
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Immunologia e infezioni
T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing
Please note that all translations are automatically generated. Click here for the English version.

T- en B-celreceptor immuunrepertoireanalyse met behulp van next-generation sequencing

7,752 Views

•

08:59 min

•

January 12, 2021

DOI:

10.3791/61792-v

DOI:
10.3791/61792-v

•

08:59 min
January 12, 2021

•

6 Views
, , , ,
1Pediatric Gastroenterology Unit, Edmond and Lily Safra Children’s Hospital,Sheba Medical Center, 2Sackler Faculty of Medicine,Tel Aviv University, 3Cancer Research Center,Sheba Medical Center, 4Molecular Hematology Laboratory,Sheba Medical Center

Summary

Automatically generated

Het huidige protocol beschrijft een methode voor DNA-isolatie van bloedmonsters en darmbiopten, generatie van TCRβ- en IGH PCR-bibliotheken voor de volgende generatie sequencing, prestaties van een NGS-run en basisgegevensanalyse.

Explore More Videos

T Cell ReceptorB Cell ReceptorNext-generation SequencingLymphocyte DiversityBiomarkersDNA IsolationCell LysisProteinase KProtein PrecipitationDNA Extraction

Read Article

Cite this Article

Werner, L., Dor, C., Salamon, N., Nagar, M., Shouval, D. S. T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing. J. Vis. Exp. (167), e61792, doi:10.3791/61792 (2021).

Download .ris file

Copy

Share Video

.

Copy