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Immunology and Infection

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JoVE Journal Immunology and Infection
T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing
 

T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing

Article DOI: 10.3791/61792-v • 08:59 min • January 12th, 2021
January 12th, 2021 •
1,2, 3,4, 1, 3,4, 1,2
1Pediatric Gastroenterology Unit, Edmond and Lily Safra Children's Hospital, Sheba Medical Center, 2Sackler Faculty of Medicine, Tel Aviv University, 3Cancer Research Center, Sheba Medical Center, 4Molecular Hematology Laboratory, Sheba Medical Center

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The current protocol describes a method for DNA isolation from blood samples and intestinal biopsies, generation of TCRβ and IGH PCR libraries for next-generation sequencing, performance of a NGS run and basic data analysis.

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T Cell Receptor B Cell Receptor Immune Repertoire Analysis Next-generation Sequencing Nucleotide Level Amino Acid Level Lymphocyte Populations Diversity Parameters Unique T Cell Clones Biomarkers Digestion Cell Lysis Intestinal Biopsies Frozen Biopsies Nuclei Lysis Solution Proteinase K DNA Isolation Blood
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