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T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing
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Journal JoVE Immunologie et infection
T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing

T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing

7,752 Views

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08:59 min

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January 12, 2021

DOI:

10.3791/61792-v

DOI:
10.3791/61792-v

•

08:59 min
January 12, 2021

•

6 Views
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1Pediatric Gastroenterology Unit, Edmond and Lily Safra Children’s Hospital,Sheba Medical Center, 2Sackler Faculty of Medicine,Tel Aviv University, 3Cancer Research Center,Sheba Medical Center, 4Molecular Hematology Laboratory,Sheba Medical Center

Summary

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The current protocol describes a method for DNA isolation from blood samples and intestinal biopsies, generation of TCRβ and IGH PCR libraries for next-generation sequencing, performance of a NGS run and basic data analysis.

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T Cell ReceptorB Cell ReceptorNext-generation SequencingLymphocyte DiversityBiomarkersDNA IsolationCell LysisProteinase KProtein PrecipitationDNA Extraction

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Cite this Article

Werner, L., Dor, C., Salamon, N., Nagar, M., Shouval, D. S. T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing. J. Vis. Exp. (167), e61792, doi:10.3791/61792 (2021).

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