This protocol describes the use of Asymmetrical Flow Field-Flow Fractionation coupled with UV-vis detection for the determination of the size of an unknown gold nanoparticle sample.
Drexel, R., Sogne, V., Dinkel, M., Meier, F., Klein, T. Asymmetrical Flow Field-Flow Fractionation for Sizing of Gold Nanoparticles in Suspension. J. Vis. Exp. (163), e61757, doi:10.3791/61757 (2020).