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单分子鱼的转录活性等位基因单细胞分析

JoVE Journal
Biochimica

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JoVE Journal Biochimica

Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH

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单分子鱼的转录活性等位基因单细胞分析

2,994 Views

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06:26 min

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September 20, 2020

DOI:

10.3791/61680-v

DOI:

10.3791/61680-v

•

06:26 min

September 20, 2020

•

4 Views

Ragini M. Mistry^1,2, Pankaj K. Singh^1,3, Maureen G. Mancini^1,2, Fabio Stossi^1,2, Michael A. Mancini^1,2,3,4

¹GCC Center for Advanced Microscopy and Image Informatics, ²Department of Molecular and Cellular Biology,Baylor College of Medicine, ³Center for Translational Cancer Research, Institute of Biosciences and Technology,Texas A&M University, ⁴Department of Pharmacology and Chemical Biology,Baylor College of Medicine

Summary

单分子RNA荧光就地杂交（smFISH）是一种在单细胞水平上准确量化特定RNA水平和定位的方法。在这里，我们报告我们已验证的实验室协议，用于湿板凳处理、成像和图像分析，用于特定RNA的单细胞量化。

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