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Calcium Imaging in Freely Behaving Caenorhabditis elegans with Well-Controlled, Nonlocalized Vibration
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Calcium Imaging in Freely Behaving Caenorhabditis elegans with Well-Controlled, Nonlocalized Vibration
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06:50 min
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April 29, 2021
DOI:
1Program of Biomedical Science, Graduate School of Integrated Sciences for Life,Hiroshima University, 2Institute for Quantum Life Science,National Institute for Quantum and Radiological Science and Technology, 3National Institute for Radiological Sciences,National Institute for Quantum and Radiological Science and Technology, 4JST, PRESTO
Summary
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Reported here is a system for calcium imaging in freely behaving Caenorhabditis elegans with well-controlled, nonlocalized vibration. This system allows researchers to evoke nonlocalized vibrations with well-controlled properties at nano-scale displacement and to quantify calcium currents during responses of C. elegans to the vibrations.
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Shigyou, K., Maeoka, H., Igarashi, R., Sugi, T. Calcium Imaging in Freely Behaving Caenorhabditis elegans with Well-Controlled, Nonlocalized Vibration. J. Vis. Exp. (170), e61626, doi:10.3791/61626 (2021).
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