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用于大规模表观遗传学研究的半自动ChIP-Seq程序
JoVE Journal
Biologia
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JoVE Journal Biologia
A Semiautomated ChIP-Seq Procedure for Large-scale Epigenetic Studies
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用于大规模表观遗传学研究的半自动ChIP-Seq程序

DOI:
10.3791/61617-v

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08:04 min

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August 13, 2020

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, , , , ,
1Division of Vaccine Discovery,La Jolla Institute for Immunology

Capitoli

  • 00:05Introduction
  • 00:26Chromatin Shearing
  • 01:28Automated Chromatin Immunoprecipitation Sequencing (ChIP-Seq)
  • 03:03Library Adaptor Transposase Integration
  • 03:49Tagmented DNA Fragment Purification
  • 04:46Purified Sample Amplification and Size-Selection
  • 05:44Results: Representative Semiautomated ChIP-Seq
  • 07:27Conclusion

Summary

Traduzione automatica

Traduzione automatica

本文描述了使用ChIP液体处理平台对与特定组蛋白修饰（H3K27ac）相关的DNA区域的半自动，微量尺度的ChIP-Seq方案，然后使用标记进行文库制备。该程序包括出于质量和数量目的的对照评估，并且可以用于其他组蛋白修饰或转录因子。

Tags

ChIP-SeqEpigenetic StudiesChromatin ShearingHistone ModificationProtein A BeadsTagmentationHigh-throughputSemiautomatedReproducibilityHands-on Time Reduction

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