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Three-Dimensional Motor Nerve Organoid Generation
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JoVE Journal Neuroscienze
Three-Dimensional Motor Nerve Organoid Generation

Three-Dimensional Motor Nerve Organoid Generation

8,225 Views

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09:57 min

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September 24, 2020

DOI:

10.3791/61544-v

DOI:
10.3791/61544-v

•

09:57 min
September 24, 2020

•

61 Views
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1Institute of Industrial Science,The University of Tokyo, 2Department of Chemistry and Biotechnology, School of Engineering,The University of Tokyo, 3Faculty of Science and Engineering,Tecnologico de Monterrey, 4Jiksak Bioengineering, Inc.

Summary

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This protocol provides a comprehensive procedure to fabricate human iPS cell-derived motor nerve organoid through spontaneous assembly of a robust bundle of axons extended from a spheroid in a tissue culture chip.

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Cite this Article

Osaki, T., Chow, S. Y. A., Nakanishi, Y., Hernández, J., Kawada, J., Fujii, T., Ikeuchi, Y. Three-Dimensional Motor Nerve Organoid Generation. J. Vis. Exp. (163), e61544, doi:10.3791/61544 (2020).

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