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قياس حجم عفوية Ca2 + التدفقات وآثارها المصب في الخلايا العصبية الماوس الأولية Midbrain
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
Quantifying Spontaneous Ca2+ Fluxes and their Downstream Effects in Primary Mouse Midbrain Neurons
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قياس حجم عفوية Ca2 + التدفقات وآثارها المصب في الخلايا العصبية الماوس الأولية Midbrain

5,822 Views

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06:54 min

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September 09, 2020

DOI:

10.3791/61481-v

DOI:
10.3791/61481-v

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06:54 min
September 09, 2020

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3 Views
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1Department of Neuroscience and Experimental Therapeutics,Texas A&M University Health Science Center, College of Medicine, 2Texas A&M Institute for Neuroscience

Tags

Calcium FluxesDopaminergic NeuronsParkinson’s DiseaseNeuroprotective CompoundsPrimary Midbrain NeuronsCalcium-mediated ApoptosisGCaMP6fHEPES Recording BufferGlutamateNBQXConfocal MicroscopyPeristaltic Pump

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Bancroft, E. A., Srinivasan, R. Quantifying Spontaneous Ca2+ Fluxes and their Downstream Effects in Primary Mouse Midbrain Neurons. J. Vis. Exp. (163), e61481, doi:10.3791/61481 (2020).

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