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Journal / Neuroscience / Quantifying Spontaneous Ca2+ Fluxes and their…
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Neuroscience

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JoVE Journal Neuroscience
Quantifying Spontaneous Ca2+ Fluxes and their Downstream Effects in Primary Mouse Midbrain Neurons
 

Quantifying Spontaneous Ca2+ Fluxes and their Downstream Effects in Primary Mouse Midbrain Neurons

Article DOI: 10.3791/61481-v • 06:54 min • September 9th, 2020
September 9th, 2020 •
1, 1,2
1Department of Neuroscience and Experimental Therapeutics, Texas A&M University Health Science Center, College of Medicine, 2Texas A&M Institute for Neuroscience

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Here we present a protocol to measure in vitro Ca2+ fluxes in midbrain neurons and their downstream effects on caspase-3 using primary mouse midbrain cultures. This model can be employed to study pathophysiologic changes related to abnormal Ca2+ activity in midbrain neurons, and to screen novel therapeutics for anti-apoptotic properties.

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Keywords: Calcium Fluxes Dopaminergic Neurons Parkinson's Disease Neuroprotective Compounds Primary Midbrain Neurons Calcium-mediated Apoptosis GCaMP6f HEPES Recording Buffer Glutamate NBQX Confocal Microscopy Peristaltic Pump
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