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从成人人类脑组织获取人类微胶质
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Neuroscienze
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JoVE Journal Neuroscienze
Obtaining Human Microglia from Adult Human Brain Tissue
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从成人人类脑组织获取人类微胶质

DOI:
10.3791/61438-v

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09:41 min

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August 30, 2020

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, , , ,
1Department of Bioscience and Bioengineering,Indian Institute of Technology Jodhpur, 2Department of Neurosurgery,All India Institute of Medical Sciences Jodhpur

Capitoli

  • 00:00Introduction
  • 01:06Tissue Acquisition and Processing: Day 0
  • 05:35Cell Culture: Day 2
  • 07:59Cell Culture: Day 4
  • 08:26Results: Representative Images of Adult Human Microglia
  • 09:15Conclusion

Summary

Traduzione automatica

Traduzione automatica

该协议是一种高效、经济、稳健的方法，将原发性微胶质与活的、成人的、人类脑组织隔离在一起。分离的原人类微胶质可以作为研究平衡和疾病细胞过程的工具。

Tags

Human MicrogliaPrimary Microglia CellsImmunocytochemistryCell IsolationTrypsin-EDTACentrifugationCell Culture

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