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Obtaining Human Microglia from Adult Human Brain Tissue
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神经科学
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JoVE 杂志 神经科学
Obtaining Human Microglia from Adult Human Brain Tissue

Obtaining Human Microglia from Adult Human Brain Tissue

6,852 Views

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09:41 min

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August 30, 2020

DOI:

10.3791/61438-v

DOI:
10.3791/61438-v

•

09:41 min
August 30, 2020

•

5 Views
, , , ,
1Department of Bioscience and Bioengineering,Indian Institute of Technology Jodhpur, 2Department of Neurosurgery,All India Institute of Medical Sciences Jodhpur

Summary

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This protocol is an efficient, cost effective and robust method of isolating primary microglia from live, adult, human brain tissue. Isolated primary human microglia can serve as a tool for studying cellular processes in homeostasis and disease.

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Agrawal, I., Saxena, S., Nair, P., Jha, D., Jha, S. Obtaining Human Microglia from Adult Human Brain Tissue. J. Vis. Exp. (162), e61438, doi:10.3791/61438 (2020).

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