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Quantitation of Rabies Virus in Various Bovine Brain Structures
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Quantitation of Rabies Virus in Various Bovine Brain Structures

Quantitation of Rabies Virus in Various Bovine Brain Structures

DOI:
10.3791/61429-v

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13:42 min

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May 22, 2021

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, , ,
1Centro Nacional de Investigación Disciplinaria en Microbiología Animal (CENID-MA), INIFAP, 2Campus Toluca,Universidad del Valle de México, 3Facultad de Ciencias Naturales,Universidad Autónoma de Querétaro

Capitoli

  • 00:00Introduction
  • 01:31Protocols
  • 09:05Representative Results
  • 12:02Discussion and Conclusion

Summary

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This protocol presents a qRT-PCR-based approach for determining the rabies virus nucleoprotein (N) gene copy number within various bovine brain anatomical structures using in vitro transcription.

Tags

Rabies VirusQuantitationBovine Brain StructuresPCRQRT-PCRNucleoprotein N GeneRNA ExtractionGuanidinium IsothiocyanateChloroformIsopropyl AlcoholEthanol

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