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使用组合前体同位素标签和同位素标记 （cPILOT） 实现自动样品多路复用
JoVE Journal
Chimica
This content is Free Access.
JoVE Journal Chimica
Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)
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使用组合前体同位素标签和同位素标记 （cPILOT） 实现自动样品多路复用

5,208 Views

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09:24 min

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December 18, 2020

DOI:

10.3791/61342-v

DOI:
10.3791/61342-v

•

09:24 min
December 18, 2020

•

2 Views
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1Department of Chemistry,Vanderbilt University, 2Vanderbilt Memory & Alzheimer’s Center,Vanderbilt University Medical Center, 3Vanderbilt Brain Institute,Vanderbilt University Medical Center, 4Vanderbilt Institute of Chemical Biology,Vanderbilt University Medical Center

Summary

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组合前体同位素标记和同位素标记 （cPILOT） 是一种增强的样品多路复用策略，能够增加可与可用同位素标记同时分析的样本数量。机器人平台的整合大大提高了实验的吞吐量、可重复性和定量精度。

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Cite this Article

Arul, A. B., Robinson, R. A. Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT). J. Vis. Exp. (166), e61342, doi:10.3791/61342 (2020).

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