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研究伤口引起的多倍体化的果蝇模型
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
A Drosophila Model to Study Wound-induced Polyploidization
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研究伤口引起的多倍体化的果蝇模型

DOI:
10.3791/61252-v

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07:27 min

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June 09, 2020

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, , , ,
1Biology Department,Boston College, 2Graduate School of Biomedical Sciences and Engineering and Kathryn W. Davis Center for Regenerative Biology and Medicine, MDI Biological Laboratory,University of Maine

Capitoli

  • 00:04Introduction
  • 00:32Adult Fruit Fly Staging and Wounding
  • 01:49Fly Abdomen Dissection
  • 04:00Stained Tissue Mounting
  • 04:57Results: Representative Re-Epithelization and Endoreplication Detection
  • 06:47Conclusion

Summary

Traduzione automatica

Traduzione automatica

伤口诱导的多倍体化是一种保守的组织修复策略，其中细胞在大小上生长，而不是分裂，以弥补细胞损失。下面是一个详细的协议，如何使用果蝇作为模型来测量皮骨及其遗传调控在上皮伤口修复。

Tags

Keywords: DrosophilaWound-induced PolyploidizationEpithelial CellsPloidyAbdominal WoundingGrace’s SolutionDissectionMicroscopy

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