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High-Throughput Assays of Critical Thermal Limits in Insects
Capitoli
- 00:04Introduction
- 00:40Critical Thermal Minima (CTmin) Assay Setup
- 01:29Critical Thermal Minima (CTmin) Assay
- 03:46High-Throughput Heat Knockdown (KD) Assay Setup
- 04:19High-Throughput Heat Knockdown (KD) Assay
- 05:45Results: Representative Lower and Upper Thermal Limits of Select Lines from theDrosophila Genetic Reference Panel (DGRP)
- 06:16Conclusion
Thermal limits can predict the environments organisms tolerate, which is valuable information in the face of rapid climate change. Described here are high-throughput protocols to assess critical thermal minima and heat knockdown time in insects. Both protocols maximize the throughput and minimize the cost of the assays.
