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Isolating Central Nervous System Tissues and Associated Meninges for the Downstream Analysis of Immune cells
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Isolating Central Nervous System Tissues and Associated Meninges for the Downstream Analysis of Immune cells

Isolating Central Nervous System Tissues and Associated Meninges for the Downstream Analysis of Immune cells

DOI:
10.3791/61166-v

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09:35 min

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May 19, 2020

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, , , , ,
1Department of Neurology, Geisel School of Medicine,Dartmouth-Hitchcock Medical Center, 2Program in Experimental and Molecular Medicine,Dartmouth College

Capitoli

  • 00:05Introduction
  • 00:53Processing Brain and Spinal Cord Samples
  • 02:01Preparation of the Meninges and CNS Tissues for Flow Cytometry Staining
  • 04:33Preparation of Single‐Cell Suspensions of Brain and Spinal Cord Tissue
  • 06:57Results: Immune Cells in CNS Tissues of TMEV‐IDD Mice
  • 08:50Conclusion

Summary

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This paper presents two optimized protocols for examining resident and peripherally derived immune cells within the central nervous system, including the brain, spinal cord, and meninges. Each of these protocols helps to ascertain the function and composition of the cells occupying these compartments under steady state and inflammatory conditions.

Tags

MeningesImmune CellsSingle Cell AnalysisHistological MethodsMicrogliaAstrocytesPericytesEndothelial CellsStromal CellsNeuronsBrainSpinal CordDecalcificationSkull CapVertebrae Column

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