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Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure
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JoVE Journal Bioingegneria
Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure

Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure

DOI:
10.3791/61141-v

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08:25 min

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June 05, 2020

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, , , , , ,
1In vitro Toxicology Group,Swansea University Medical School

Capitoli

  • 00:04Introduction
  • 00:49HepG2 Spheroid Preparation
  • 02:50Nanomaterial/Chemical Exposure and Spheroid Harvesting
  • 04:45Micronucleus Assay and Scoring
  • 05:55Results: Morphology and Characteristics of HepG2 Spheroids
  • 07:40Conclusion

Summary

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Traduzione automatica

This procedure was established to be used for developing advanced 3D hepatic cultures in vitro, which can provide a more physiologically relevant assessment of the genotoxic hazards associated with nanomaterial exposures over both an acute or long-term, repeated dose regimes.

Tags

Keywords: 3D Liver ModelHep G2 SpheroidIn Vitro GenotoxicityLong-term Nanomaterial ExposureAlternative To Animal TestingMicronucleus AssayCell SeedingMedia ChangeAgaroseCentrifugationEngineered Nanomaterials (ENMs)

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